Rapid Continuous Electrical Lysis of Bacteria on Structured Electrodes Preserves Rna Integrity

نویسندگان

  • Mahla Poudineh
  • Reza M. Mohamadi
  • Andrew Sage
  • Laili Mahmoudian
  • Edward H. Sargent
  • Shana O. Kelley
چکیده

We present a rapid electrical lysis method using structured gold electrodes in a microfluidic device. This strategy is capable of efficient and high throughput lysis of bacteria to prepare intracellular genomic materials for subsequent analysis. High electrical field at the tips of structured gold electrodes eliminates the requirement of high voltage. We have used AC voltage for lysis, and shown at low frequency; lysis would be electrochemical while at high frequency it occurs due to the electrical field.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Correction: Use of Surface Enhanced Blocking (SEB) Electrodes for Microbial Cell Lysis in Flow-Through Devices

By simultaneously subjecting microbial cells to high amplitude pulsed electric fields and flash heating of the cell suspension fluid, effective release of intracellular contents was achieved. The synergistic effect of the applied electric field and elevated temperature on cell lysis in a flow-through device was demonstrated for Gram-negative and Gram-positive bacteria, and Mycobacterium species...

متن کامل

Continuous-flow electrical lysis device with integrated control by dielectrophoretic cell sorting.

We present a device capable of electrical cell lysis and evaluation of lysis efficiency in continuous flow using dielectrophoretic cell sorting. We use a combination of AC electrical fields and so-called liquid electrodes to avoid bubble creation at the electrode surface. The electrical field distribution is calculated in different electrode configurations by numerical simulations. Cell sorting...

متن کامل

Fabrication of Resistive E. coli Biosensor Based on ZnO Nanorods and Nanoparticles

In this paper, a biosensor has been fabricated using ZnO nanorods and nanoparticles to detect different concentrations of the E. coli bacteria. The innovation of this paper lies in design and fabrication of the resistive type E Coli bacteria sensor. To make this biosensors, printed circuit board based electrodes are designed and made in an interdigitated shape. Both hydrothermal and drop cast m...

متن کامل

Evaluation of isolation methods and RNA integrity for bacterial RNA quantitation.

RNA integrity is critical for successful RNA quantitation for mammalian tissues, but the level of integrity required differs among tissues. The level of integrity required for quantitation has not been determined for bacterial RNA. Three RNA isolation methods were evaluated for their ability to produce high quality RNA from Dickeya dadantii, a bacterium refractory to RNA isolation. Bacterial ly...

متن کامل

Use of electrochemical DNA biosensors for rapid molecular identification of uropathogens in clinical urine specimens.

We describe the first species-specific detection of bacterial pathogens in human clinical fluid samples using a microfabricated electrochemical sensor array. Each of the 16 sensors in the array consisted of three single-layer gold electrodes-working, reference, and auxiliary. Each of the working electrodes contained one representative from a library of capture probes, each specific for a clinic...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2014